Structure-Function Relationship of a Novel PR-5 Protein with Antimicrobial Activity from Soy Hulls.
Identifieur interne : 000B25 ( Main/Exploration ); précédent : 000B24; suivant : 000B26Structure-Function Relationship of a Novel PR-5 Protein with Antimicrobial Activity from Soy Hulls.
Auteurs : Chun Liu [République populaire de Chine] ; Fenfen Cheng [République populaire de Chine] ; Yingen Sun [République populaire de Chine] ; Hongyu Ma [République populaire de Chine] ; Xiaoquan Yang [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2016.
Descripteurs français
- KwdFr :
- Anti-infectieux (composition chimique), Anti-infectieux (pharmacologie), Données de séquences moléculaires (MeSH), Phytophthora (croissance et développement), Phytophthora (effets des médicaments et des substances chimiques), Protéines de soja (composition chimique), Protéines de soja (pharmacologie), Pseudomonas syringae (croissance et développement), Pseudomonas syringae (effets des médicaments et des substances chimiques), Relation structure-activité (MeSH), Similitude de séquences d'acides aminés (MeSH), Simulation de docking moléculaire (MeSH), Soja (composition chimique), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Anti-infectieux, Protéines de soja, Soja.
- croissance et développement : Phytophthora, Pseudomonas syringae.
- effets des médicaments et des substances chimiques : Phytophthora, Pseudomonas syringae.
- pharmacologie : Anti-infectieux, Protéines de soja.
- Données de séquences moléculaires, Relation structure-activité, Similitude de séquences d'acides aminés, Simulation de docking moléculaire, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Anti-Infective Agents (chemistry), Anti-Infective Agents (pharmacology), Molecular Docking Simulation (MeSH), Molecular Sequence Data (MeSH), Phytophthora (drug effects), Phytophthora (growth & development), Pseudomonas syringae (drug effects), Pseudomonas syringae (growth & development), Sequence Homology, Amino Acid (MeSH), Soybean Proteins (chemistry), Soybean Proteins (pharmacology), Soybeans (chemistry), Structure-Activity Relationship (MeSH).
- MESH :
- chemical , chemistry : Anti-Infective Agents, Soybean Proteins.
- chemical , pharmacology : Anti-Infective Agents, Soybean Proteins.
- chemistry : Soybeans.
- drug effects : Phytophthora, Pseudomonas syringae.
- growth & development : Phytophthora, Pseudomonas syringae.
- Amino Acid Sequence, Molecular Docking Simulation, Molecular Sequence Data, Sequence Homology, Amino Acid, Structure-Activity Relationship.
Abstract
An alkaline isoform of the PR-5 protein (designated GmOLPc) has been purified from soybean hulls and identified by MALDI-TOF/TOF-MS. GmOLPc effectively inhibited in vitro the growth of Phytophthora soja spore and Pseudomonas syringae pv glycinea. The antimicrobial activity of GmOLPc should be mainly ascribed to its high binding affinity with vesicles composed of DPPG, (1,3)-β-D-glucans, and weak endo-(1,3)-β-D-glucanase activity. From the 3D models, predicted by the homology modeling, GmOLPc contains an extended negatively charged cleft. The cleft was proved to be a prerequisite for endo-(1,3)-β-D-glucanase activity. Molecular docking revealed that the positioning of linear (1,3)-β-D-glucans in the cleft of GmOLPc allowed an interaction with Glu83 and Asp101 that were responsible for the hydrolytic cleavage of glucans. Interactions of GmOLPc with model membranes indicated that GmOLPc possesses good surface activity which could contribute to its antimicrobial activity, as proved by the behavior of perturbing the integrity of membranes through surface hydrophobic amino acid residues (Phe89 and Phe94).
DOI: 10.1021/acs.jafc.5b04771
PubMed: 26753535
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structure-Function Relationship of a Novel PR-5 Protein with Antimicrobial Activity from Soy Hulls.</title><author><name sortKey="Liu, Chun" sort="Liu, Chun" uniqKey="Liu C" first="Chun" last="Liu">Chun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Cheng, Fenfen" sort="Cheng, Fenfen" uniqKey="Cheng F" first="Fenfen" last="Cheng">Fenfen Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Sun, Yingen" sort="Sun, Yingen" uniqKey="Sun Y" first="Yingen" last="Sun">Yingen Sun</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Ma, Hongyu" sort="Ma, Hongyu" uniqKey="Ma H" first="Hongyu" last="Ma">Hongyu Ma</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University , Nanjing 210095, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University , Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiaoquan" sort="Yang, Xiaoquan" uniqKey="Yang X" first="Xiaoquan" last="Yang">Xiaoquan Yang</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26753535</idno><idno type="pmid">26753535</idno><idno type="doi">10.1021/acs.jafc.5b04771</idno><idno type="wicri:Area/Main/Corpus">000C96</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C96</idno><idno type="wicri:Area/Main/Curation">000C96</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000C96</idno><idno type="wicri:Area/Main/Exploration">000C96</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Structure-Function Relationship of a Novel PR-5 Protein with Antimicrobial Activity from Soy Hulls.</title><author><name sortKey="Liu, Chun" sort="Liu, Chun" uniqKey="Liu C" first="Chun" last="Liu">Chun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Cheng, Fenfen" sort="Cheng, Fenfen" uniqKey="Cheng F" first="Fenfen" last="Cheng">Fenfen Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Sun, Yingen" sort="Sun, Yingen" uniqKey="Sun Y" first="Yingen" last="Sun">Yingen Sun</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Ma, Hongyu" sort="Ma, Hongyu" uniqKey="Ma H" first="Hongyu" last="Ma">Hongyu Ma</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University , Nanjing 210095, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University , Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiaoquan" sort="Yang, Xiaoquan" uniqKey="Yang X" first="Xiaoquan" last="Yang">Xiaoquan Yang</name><affiliation wicri:level="1"><nlm:affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou 510640, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Anti-Infective Agents (chemistry)</term><term>Anti-Infective Agents (pharmacology)</term><term>Molecular Docking Simulation (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phytophthora (drug effects)</term><term>Phytophthora (growth & development)</term><term>Pseudomonas syringae (drug effects)</term><term>Pseudomonas syringae (growth & development)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Soybean Proteins (chemistry)</term><term>Soybean Proteins (pharmacology)</term><term>Soybeans (chemistry)</term><term>Structure-Activity Relationship (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anti-infectieux (composition chimique)</term><term>Anti-infectieux (pharmacologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (effets des médicaments et des substances chimiques)</term><term>Protéines de soja (composition chimique)</term><term>Protéines de soja (pharmacologie)</term><term>Pseudomonas syringae (croissance et développement)</term><term>Pseudomonas syringae (effets des médicaments et des substances chimiques)</term><term>Relation structure-activité (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Simulation de docking moléculaire (MeSH)</term><term>Soja (composition chimique)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anti-Infective Agents</term><term>Soybean Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Infective Agents</term><term>Soybean Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Anti-infectieux</term><term>Protéines de soja</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phytophthora</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Anti-infectieux</term><term>Protéines de soja</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Molecular Docking Simulation</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Relation structure-activité</term><term>Similitude de séquences d'acides aminés</term><term>Simulation de docking moléculaire</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An alkaline isoform of the PR-5 protein (designated GmOLPc) has been purified from soybean hulls and identified by MALDI-TOF/TOF-MS. GmOLPc effectively inhibited in vitro the growth of Phytophthora soja spore and Pseudomonas syringae pv glycinea. The antimicrobial activity of GmOLPc should be mainly ascribed to its high binding affinity with vesicles composed of DPPG, (1,3)-β-D-glucans, and weak endo-(1,3)-β-D-glucanase activity. From the 3D models, predicted by the homology modeling, GmOLPc contains an extended negatively charged cleft. The cleft was proved to be a prerequisite for endo-(1,3)-β-D-glucanase activity. Molecular docking revealed that the positioning of linear (1,3)-β-D-glucans in the cleft of GmOLPc allowed an interaction with Glu83 and Asp101 that were responsible for the hydrolytic cleavage of glucans. Interactions of GmOLPc with model membranes indicated that GmOLPc possesses good surface activity which could contribute to its antimicrobial activity, as proved by the behavior of perturbing the integrity of membranes through surface hydrophobic amino acid residues (Phe89 and Phe94).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26753535</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>05</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>64</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>Feb</Month><Day>03</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Structure-Function Relationship of a Novel PR-5 Protein with Antimicrobial Activity from Soy Hulls.</ArticleTitle><Pagination><MedlinePgn>948-59</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.5b04771</ELocationID><Abstract><AbstractText>An alkaline isoform of the PR-5 protein (designated GmOLPc) has been purified from soybean hulls and identified by MALDI-TOF/TOF-MS. GmOLPc effectively inhibited in vitro the growth of Phytophthora soja spore and Pseudomonas syringae pv glycinea. The antimicrobial activity of GmOLPc should be mainly ascribed to its high binding affinity with vesicles composed of DPPG, (1,3)-β-D-glucans, and weak endo-(1,3)-β-D-glucanase activity. From the 3D models, predicted by the homology modeling, GmOLPc contains an extended negatively charged cleft. The cleft was proved to be a prerequisite for endo-(1,3)-β-D-glucanase activity. Molecular docking revealed that the positioning of linear (1,3)-β-D-glucans in the cleft of GmOLPc allowed an interaction with Glu83 and Asp101 that were responsible for the hydrolytic cleavage of glucans. Interactions of GmOLPc with model membranes indicated that GmOLPc possesses good surface activity which could contribute to its antimicrobial activity, as proved by the behavior of perturbing the integrity of membranes through surface hydrophobic amino acid residues (Phe89 and Phe94).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Chun</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Fenfen</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Yingen</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Hongyu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University , Nanjing 210095, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiaoquan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou 510640, People's Republic of China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>01</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000890">Anti-Infective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D030262">Soybean Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000890" MajorTopicYN="N">Anti-Infective Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044224" MajorTopicYN="N">Pseudomonas syringae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030262" MajorTopicYN="N">Soybean Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013329" MajorTopicYN="N">Structure-Activity Relationship</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">antimicrobial protein</Keyword><Keyword MajorTopicYN="N">endo-(1,3)-β-d-glucanase activity</Keyword><Keyword MajorTopicYN="N">soy hulls</Keyword><Keyword MajorTopicYN="N">structure−function relationship</Keyword><Keyword MajorTopicYN="N">surface activity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>1</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>1</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26753535</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.5b04771</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Liu, Chun" sort="Liu, Chun" uniqKey="Liu C" first="Chun" last="Liu">Chun Liu</name></region><name sortKey="Cheng, Fenfen" sort="Cheng, Fenfen" uniqKey="Cheng F" first="Fenfen" last="Cheng">Fenfen Cheng</name><name sortKey="Ma, Hongyu" sort="Ma, Hongyu" uniqKey="Ma H" first="Hongyu" last="Ma">Hongyu Ma</name><name sortKey="Sun, Yingen" sort="Sun, Yingen" uniqKey="Sun Y" first="Yingen" last="Sun">Yingen Sun</name><name sortKey="Yang, Xiaoquan" sort="Yang, Xiaoquan" uniqKey="Yang X" first="Xiaoquan" last="Yang">Xiaoquan Yang</name><name sortKey="Yang, Xiaoquan" sort="Yang, Xiaoquan" uniqKey="Yang X" first="Xiaoquan" last="Yang">Xiaoquan Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B25 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B25 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26753535
|texte= Structure-Function Relationship of a Novel PR-5 Protein with Antimicrobial Activity from Soy Hulls.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26753535" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |